Mass spec calibration solutions thermo fisher scientific. Can anyone help with an ltq orbitrap xl mass spectrometer. Lipid analysis untargeted lipidomics, shotgun lipidomics. Thermo scientific orbitrap elite hybrid ion traporbitrap mass spectrometer. Ltq orbitrap velos is a hybrid mass spectrometer incorporating the ltq velos dual cell linear trap and the orbitrap analyzer.
Instead, set the system to full scan mode before initiating calibration. The high mass accuracy of the ltq orbitrap velos increases. B, the dual linear ion trap design enables efficient trapping and activation in the highpressure cell left and fast scanning and detection in the low pressure cell. Once the machine become functional, operating demo and introductory training of orbitrap with proxeon nanolc was provided to nii research scholars from different labs. Ltq orbitrap velos getting started thermo fisher scientific. Once you have your skyline document open on the ltq instrument control computer, you are ready to export it to a native ltq method or. Calibration function for the orbitrap ftms accounting for the. Variants include the ltq, ltq ft circa 2003, ltq orbitrap circa 2005, ltq xl electrontransfer dissociation, etd circa 2007, ltq orbitrap xl circa 2007, ltq discovery, ltq ft ultra, ltq velos circa 200910, ltq orbitrap velos circa 200910. All information in this guide concerning the orbitrap velos pro mass.
Used thermo fisher scientific ltq orbitrap velos 8002663. Ltq orbitrap velos is the next generation mass spectrometers, thanks to its significantly increased sensitivity and scan speed. The data was generated by hplc apparatus agilent 1200 series, ltq orbitrap xl thermofisher scientific. The velos pro ion trap contributes enhanced ion optics that increase sensitivity and reliability, a greater dynamic range for better precursor detection, and the power of msn identification. Thermo scientific ltq orbitrap velos hy b rid ft mass. Performance evaluation of a hybrid linear ion trap. Additional tubing extension lengths are available contact us for a. The ltqorbitrap velos and qexactive are two widely used mass spectrometers for largescale proteomic analysis. Ltq orbitrap velos is a member of the family of ltq mass spectrometer.
The ltq velos esi positive ion calibration solution can be used to calibrate the ltq velosvelos pro, the ltq orbitrap velosorbitrap velos pro, the orbitrap elite, and the qexactive mass spectrometer instruments. Protein analysis bottomup proteomics, topdown proteomics, post translational modification ptm analysis and relative quantitation. The ltq velos esi positive ion calibration solution can be used to calibrate the ltq velos velos pro, the ltq orbitrap velos orbitrap velos pro, the orbitrap elite, and the qexactive mass spectrometer instruments. Esi positive ion calibration solution, ltq, pierce standards colour reference and spectroscopy standards positive ion calibration solutions are mixtures of highly purified ionisable molecules specifically designed for positive mode calibration of ion trap and orbitrap instruments. A, the stacked ring ion guide slens increases the ion flux from the electrospray ion source into the instrument by a factor 510. The thermo ltq is a 2d linear ion trap ms, first released circa 2003. I was calibrating our ltqorbitrap xl and i noticed very unstable esi spray 40% rsd in the. Shotgun proteomics has made great advances due to the improved sensitivity, resolution, and speed of recent generations of mass spectrometers 1, 2. Thermo scientific orbitrap velos pro hybrid ms from. Tables 6 and and7 7 summarize ms and msms acquisition settings commonly employed by different groups for cid and hcd analyses performed on ltqorbitrap classic, ltqorbitrap xl, ltqorbitrap velos, orbitrap elite and q exactive. Mass spec calibration solutions thermo fisher scientific us.
Schematic of the ltq orbitrap velos ms instrument with openi. Linear trap quadropole ltq orbitrap velos mass spectrometry. Nbutylamine, caffeine, metargpheala mrfa and ultramark 1621 are used to calibrate thermo scientific ltq velos ion trap and orbitrap mass spectrometers in the esi positive ionization mode. Reference manual for the ltq velos, velos pro, ltq orbitrap velos. The esi negative ion calibration solution can be used to calibrate the ltq series, ltq velos series, the ltq orbitrap series and exactive mass spectrometer instruments. The orbitrap velos pro is a member of the family of ltq mass spectrometer ms hybrid instruments. Etd, thermo scientific easynlc ii nano lc, powervar abc15. Qexactive outperformed ltq orbitrap velos hcd in terms of number of peptide and protein groups, due to faster scan rate and higher resolution. The esi negative ion calibration solution is manufactured at an iso 9001 facility and each lot is quality controlled with strict specifications.
Department of pediatrics, medical university of vienna, vienna, austria. The orbitrap velos pro mass spectrometer is a member of the family of ltq mass spectrometer ms detectors. The high mass accuracy of the ltq orbitrap velos increases the speed and con. Ltq orbitrap velos getting started concordia university. Mass spectrum of enolase acquired at 7500 resolving power. Powervar, thermoflex chiller, computer and software, vacuum pumps, all cables. Ions generated by api are collected in the ltq xl followed by axial ejection to the cshaped storage trap which is used to store and collisionally. Esi positive ion calibration solution, ltq, pierce vwr.
A tube lens of 110 v ltq orbitrap xl instrument or slens of 50% ltq orbitrap velos instrument was used in combination with 10 v of source voltage. The thermo scientific orbitrap elite ms is the industryleading high performance hybrid mass spectrometer combining the novel highfield orbitrap analyzer with the latest dualpressure linear ion trap, the thermo scientific velos pro. The three major novel hardware elements are i the introduction of a more efficient ion transfer system stacked ring ion guide or slens, ii a dual pressure ion trap, and iii a more efficient hcd cell fig. Ltq orbitrap velos with etd thermo fisher scientific applications. The pierce ltq velos esi positive ion calibration solution is a readytouse liquid formulation ideal for quickly performing the routine calibration required to maintain the robust performance of thermo scientific mass spectrometers. The ltq orbitrap velos instrument described in this paper is a further development of the ltq orbitrap product 10. Thermo scientific pierce ltq velos esi positive ion. Jan 15, 20 the ltq orbitrap velos and qexactive mass spectrometers were compared for shotgun proteomic analysis of 1 to 1,000 ng raw 264.
The latter injects pulsed ion beams into a rapidly changing electric field in the orbitrap wherein they are trapped at high kinetic energies around an inner electrode. To accomplish this on ltq orbitrap, ltq orbitrap xl, and ltq orbitrap discovery mass spectrometers, a toggle for analysis of highmolecularweight molecules is accessible from the instrument control window tune page in instru ment control software version 2. Ltq orbitrap xl offering outstanding mass accuracy, resolving power, dynamic range, and high sensitivity the hybrid ft mass spectrometer combines a linear ion trap ms and the orbitrap mass analyzer. More product data calibration solutions for thermo scientific mass spectrometers. The highfield orbitrap analyzer increases speed, sensitivity and dynamic range to dig deeper into your samples. Ultrafast realtime data acquisition and instrument control system. The ltq orbitrap is a high performance lcms and msn system, combining rapid ltq ion trap data acquisition with high mass accuracy orbitrap mass analysis. Click the browse button beside the template file field. Mass spectrometry analysis on the ltq orbitrap velos. Can anyone help with an orbitrap mass spectrometer calibration. Welcome to the thermo scientific orbitrap velos pro system. For the ltqorbitrap velos, the mass spectrum is acquired with an orbitrap, and tandem mass. Jul 05, 20 tables 6 and and7 7 summarize ms and msms acquisition settings commonly employed by different groups for cid and hcd analyses performed on ltq orbitrap classic, ltq orbitrap xl, ltq orbitrap velos, orbitrap elite and q exactive.
Ltq orbitrap currently the orbitrap analyzer exists in two variants. This is achieved by a vacuum interface using a stacked ring radio frequency ion guide with 10fold higher transfer efficiency in msms mode and 35fold in full scan spectra, by a dual pressure ion trap. This screwed up the software running in the mass spectrometer. It couples a linear ion trap mass spectrometer to an orbitrap mass analyzer via an rfonly trapping quadrupole with a curved axis. Evaluation and optimization of mass spectrometric settings. Thermo scientific ltq orbitrap velos iet refurbished. Thermo fisher orbitrap instrument control software. Orbitrap velos pro getting started thermo fisher scientific. Analysis scheme on the velos ltq orbitrap a ms, b msddms 2 and c msddms 3.
The thermo ltq orbitrap velos mass spectrometer is a hybrid mass spectrometer incorporating the ltq velos dual cell linear trap and the orbitrap analyzer. Dremel is a registered trademark of the robert bosch tool corporation. Sample prep tools for mass spectrometry fisher scientific. Nbutylamine, caffeine, metargpheala mrfa and ultramark 1621 are used to calibrate thermo scientific ltq velos ion trap and orbitrap mass spectrometers in. About this guide this orbitrap velos pro getting started manual provides information on how to set up, calibrate, and tune the orbitrap velos pro mass spectrometer. Calibrate dialog box showing the semiautomatic page orbitrap systems.
About this guide this ltq orbitrap velos getting started manual provides information on how to set up, calibrate, and tune the ltq orbitrap velos. Calibration curve based on the number of protein groups a and number of. Orbitrap velos pro building on the industryleading thermo scientific orbitrap platform, the thermo scientific orbitrap velos pro hybrid ion traporbitrap mass spectrometer features dualpressure linear ion trap technology that delivers increased sensitivity and dynamic range with ultimate robustness. In practical traps, the outer electrode is sustained at virtual ground and a voltage of 3. The stable solution is provided in a leakproof, highpurity ptfe bottle. Dartthermo ltqorbitrap interface manual for thermo ltq orbitrap velos, ltq orbitrap xl, ltq ion trap, exactive high performance, and all other instruments that utilize the ion max source version 20120423 ionsense inc. Orbitrap eclipse ms, orbitrap fusion lumos ms, orbitrap fusion ms, and orbitrap idx ms. The ltq velos esi positive ion calibration solution is manufactured at an iso 9001 facility and each lot is quality controlled with strict specifications. Thermo scientific orbitrap velos pro hybrid ms from thermo. Thermo scientific ltq orbitrap velos hy b rid ft mass spectrometer performance speci fications performance characteristics. Calibration is not hard but still takes a while and has to be done every two weeks. Orbitrap fusion lumos, orbitrap fusion, orbitrap idx technical information update on use of pierce flexmix for calibration of orbitrap tribrid mass spectrometers.
As a result, the resolving power at mz 400 and 768 ms detection time can range from. Ltq orbitrap velos is a member of the family of ltq mass spectrometer ms hybrid instruments. A uplc system coupled with either an ltqorbitrap velos or a qexactive. These values could serve as a starting point when optimizing acquisition settings for proteomics analysis on. Low voltage safety compliance this device complies with low voltage directive 200695ec and harmonized standard iecencsaul 610101. Comparison of ltqorbitrap velos and qexactive for proteomic. Schematic of the ltq orbitrap velos ms instrument with. Ltq series getting started guide thermo fisher scientific. Orbitrap parameters via instrument control software. Currently the orbitrap analyzer exists in two variants. Foundation, ion maxs, ltq xl, orbitrap elite, orbitrap velos pro, velos, velos pro, wideband activation, and zoomscan are.
Orbitrap tribrid ms series instrument control software version 3. By combining three different extraction methods and two. Ltq xl ms emc directive 2004108ec emc compliance has been evaluated by tuv rheinland of north america, inc. Pierce ltq velos esi positive ion calibration solution. Application of ultrahigh pressure liquid chromatography.
Design and performance of a novel hybrid mass spectrometer is described. While there are two positive solutions, there is only one negative solution. Schematic of the ltq orbitrap velos ms instrument with three new hardware implementations. Metabolite analysis untargeted metabolomics and metabolite structural elucidation. Sample prep tools for mass spectrometry thermo scientific pierce ltq velos esi positive ion calibration solution calibrate ltq velos, ltq orbitrap and other mass spectrometers using a mixture of highly purified ionizable molecules designed for positive mode calibration. The thermo fisher scientific ltq orbitrap velos is a unique hybrid fouriertransform mass spectrometer. Orbitrap mass analyzer and detection of the transient.
The thermo scientific orbitrap elite mass spectrometer combines a dual linear ion trap velospro with a highfield orbitrap mass analyzer. Here the authors describe a next generation ltq orbitrap system termed velos, with significantly increased sensitivity and scan speed. The orbitrap velos is far one of the fastest mass spectrometers and is able to be coupled to capillary electrophoresis. It combines thermos robust orbitrap mass analyzer and the sensitive ltq velos dualpressure linear trap for a powerful ion analysis platform. Ltq orbitrap velos is a member of the family of ltq mass spectrometer ms detectors. The new ltq orbitrap xl hybrid ft mass spectrometer combines a linear ion trap ms and the orbitrap mass analyzer. If you can have a look at the instrument method editor page of the software, you.
Can anyone help with an ltq orbitrap xl mass spectrometer calibration issue. The ltq orbitrap velos was also used in the data dependent dd mode for both, ms 2 and ms 3 fragmentation. Resetting the tune and calibration parameters to their. Orbitrap velos pro building on the industryleading thermo scientific orbitrap platform, the thermo scientific orbitrap velos pro hybrid ion trap orbitrap mass spectrometer features dualpressure linear ion trap technology that delivers increased sensitivity and dynamic range with ultimate robustness. After processing peaks with xcms online tool i did annotations with different databases. All information in this guide concerning the ltq orbitrap velos also applies to the ltq orbitrap velos etd system where the etd module is physically coupled to the back of the ltq orbitrap velos. Representative mass spectrum for the thermo scientific pierce ltq velos esi positive ion calibration solution. Orbitrap velos pro hardware manual thermo fisher scientific. Additional tubing extension lengths are available contact us for a quotation. It is the ultimate protein identification and biomarker discovery platform featuring unsurpassed sensitivity in ms and msn, rapid scan rate, high mass accuracy and up to 100k resolution power. Pierce esi ion calibration solution, ltq, thermo scientific. The instrument was calibrated using an automatic routine based on a standard calibration solution containing caffeine, peptide mrfa, and ultramark 1621 sigma chemical. Procedures in chapters 14 can be pe rformed from the xcalibur tune plus window. Calibration function for the orbitrap ftms accounting for.